Journal: Cell reports

Article Title: The adiponectin-PPARγ axis in hepatic stellate cells regulates liver fibrosis

doi: 10.1016/j.celrep.2024.115165

Figure Lengend Snippet: (A–K) Control (Lrat-rtTA) and LAPNTG (Lrat-rtTA+TRE-Adipoq) mice were housed under thermoneutral conditions and fed HFD+DOX to induce liver fibrosis. (A) Experimental setup. (B) Adiponectin mRNA expression in HSCs after 2 weeks’ doxycycline. (C) Circulating adiponectin levels after 2 weeks’ doxycycline ( n = 5). (D) Body weight development. (E) OGTT after 8 weeks ( n = 5–8). (F–J) Liver mRNA expression of (F) Col1a1, (G) Col3a1, (H) Col4a4, (I) TGF-β, and (J) smooth muscle actin after 1 year under thermoneutral conditions. (K) Liver trichrome staining. Scale bar represents 100 μm. (L) Picrosirius red staining. Scale bar represents 100 μm. (M) Quantification of fibrotic area. (N–U) Control (MUP-uPA+Lrat-rTA) and MUPLAPNTG (MUP-uPA+Lrat-rtTA+TRE-Adipoq) mice were fed chow+DOX. (N) Experimental setup. (O–Q) Liver mRNA expression of (O) smooth muscle actin, (P) Col3a1, and (Q) Col4a4 after 20 weeks. (R) Liver trichrome staining after 20 weeks ( n = 10). (S) Picrosirius red staining. (T) Quantification of fibrotic area. (U) Liver tumors after 20 weeks’ HFD plus Dox600 ( n = 10). Data are displayed as mean ± SEM and analyzed by unpaired Student’s t test for all the figures: * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Doxycycline High fat diet (600 mg/kg diet) , BioServ , N/A.

Techniques: Control, Expressing, Staining

Journal: Cell reports

Article Title: The adiponectin-PPARγ axis in hepatic stellate cells regulates liver fibrosis

doi: 10.1016/j.celrep.2024.115165

Figure Lengend Snippet:

Article Snippet: Doxycycline High fat diet (600 mg/kg diet) , BioServ , N/A.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, cDNA Synthesis, SYBR Green Assay, Generated, Software, Fluorescence, Microscopy

Journal: PLoS ONE

Article Title: Effects of vitamin D on insulin resistance and myosteatosis in diet-induced obese mice

doi: 10.1371/journal.pone.0189707

Figure Lengend Snippet: Values are means ± S.E.M. of 6–14 animals per group. Statistical analysis was performed with Two-way analysis of variance with Bonferroni's post-hoc test. ### p<0.001 vs HFHS. p<0.001 HFHS groups vs Control groups from week 1, symbol are not reported in the figure.

Article Snippet: The animals were then randomly allocated to two experimental groups: mice fed a control diet or an High Fat-High Sugar (HFHS) diet (ssniff Spezialdiäten GmbH, Ferdinand-Gabriel-Weg, Germany) for 16 weeks.

Techniques: Control

Journal: PLoS ONE

Article Title: Effects of vitamin D on insulin resistance and myosteatosis in diet-induced obese mice

doi: 10.1371/journal.pone.0189707

Figure Lengend Snippet: Panel A. Oral glucose tolerance test ( OGTT). Values are means ± S.E.M. of 6–10 animals per group. Statistical analysis was performed with Two-way analysis of variance with Bonferroni's post-hoc test. *p<0.05, ***p<0.001 vs Control; # p<0.05, ## p<0.01, ### p<0.001 vs HFHS. Panel B. HOMA index. Data are expressed by medians and interquartile range of 4–6 animals randomly selected per group. Statistical analysis was performed with Kruskal–Wallis test with Dunn’s post hoc test. *p<0.05 vs Control; # p<0.05 vs HFHS.

Article Snippet: The animals were then randomly allocated to two experimental groups: mice fed a control diet or an High Fat-High Sugar (HFHS) diet (ssniff Spezialdiäten GmbH, Ferdinand-Gabriel-Weg, Germany) for 16 weeks.

Techniques: Control

Journal: PLoS ONE

Article Title: Effects of vitamin D on insulin resistance and myosteatosis in diet-induced obese mice

doi: 10.1371/journal.pone.0189707

Figure Lengend Snippet: The expression of total IRS-1 protein and its Ser 307 phosphorylation (panel A), total Akt protein and its Ser 473 phosphorylation (panel B), and total GSK-3β protein and its Ser 9 phosphorylation (panel C) were analyzed by Western blot on gastrocnemious homogenates of animals fed a control diet or HFHS diet, with or without vitamin D supplementation (7 μg/kg, 3 times per week). Densitometric analysis of the bands is expressed as relative optical density (O.D.) and normalized using the related control band. The data are expressed by medians and interquartile range of 5–8 randomly selected animals per group. Statistical analysis was performed with Kruskal–Wallis test with Dunn’s post hoc test. *p<0.05, **p<0.01 vs Control; #p<0.05, ##p<0.01 vs HFHS.

Article Snippet: The animals were then randomly allocated to two experimental groups: mice fed a control diet or an High Fat-High Sugar (HFHS) diet (ssniff Spezialdiäten GmbH, Ferdinand-Gabriel-Weg, Germany) for 16 weeks.

Techniques: Expressing, Phospho-proteomics, Western Blot, Control

Journal: PLoS ONE

Article Title: Effects of vitamin D on insulin resistance and myosteatosis in diet-induced obese mice

doi: 10.1371/journal.pone.0189707

Figure Lengend Snippet: (A) Representative western blotting analysis for the expression of NF-κB p65 subunit. Protein expression was analyzed by Western blot on cytosol and nucleus homogenates of gastrocnemious from animals fed a control diet or HFHS diet with or without vitamin D supplementation (7 μg/kg, 3 times per week). Densitometric analysis of the bands is expressed as relative optical density (O.D.) corrected for the GADPH (cytosol) or histone (nucleus) contents, and normalized using the related control band. NF-κB p65 subunit translocation was expressed as nucleus/cytosol ratio normalized using the related control band. (B) TNF-alpha levels were measured by ELISA in the mouse gastrocnemious homogenates. The data are expressed by medians and interquartile range) of 5–6 randomly selected animals per group. Statistical analysis was performed with Kruskal–Wallis test with Dunn’s post hoc test. *p<0.05 vs Control; #p<0.05 vs HFHS.

Article Snippet: The animals were then randomly allocated to two experimental groups: mice fed a control diet or an High Fat-High Sugar (HFHS) diet (ssniff Spezialdiäten GmbH, Ferdinand-Gabriel-Weg, Germany) for 16 weeks.

Techniques: Western Blot, Expressing, Control, Translocation Assay, Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Effects of vitamin D on insulin resistance and myosteatosis in diet-induced obese mice

doi: 10.1371/journal.pone.0189707

Figure Lengend Snippet: Representative photomicrographs (10x, 20x, 40x magnification) of Oil Red staining on gastrocnemious sections from animals fed a control diet or HFHS diet with or without vitamin D supplementation (7 μg/kg, 3 times per week).

Article Snippet: The animals were then randomly allocated to two experimental groups: mice fed a control diet or an High Fat-High Sugar (HFHS) diet (ssniff Spezialdiäten GmbH, Ferdinand-Gabriel-Weg, Germany) for 16 weeks.

Techniques: Staining, Control

Journal: PLoS ONE

Article Title: Effects of vitamin D on insulin resistance and myosteatosis in diet-induced obese mice

doi: 10.1371/journal.pone.0189707

Figure Lengend Snippet: (A) Representative Western blotting analysis for the expression of SCAP and active/inactive SREBP1c. Protein expression was evaluated on gastrocnemious homogenates of animals fed a control diet or HFHS diet with or without vitamin D supplementation (7 μg/kg, 3 times per week). (B) Densitometric analysis of the bands is expressed as relative optical density (O.D.), corrected for the GADPH contents, and normalized using the related control band. The data are means ± S.E.M. of 6–8 randomly selected animals per group. Statistical analysis was performed by One-way analysis of variance with Bonferroni's post-hoc test. **p<0.01, ***p<0.001 vs Control; ## p<0.01, ### p<0.001 vs HFHS.

Article Snippet: The animals were then randomly allocated to two experimental groups: mice fed a control diet or an High Fat-High Sugar (HFHS) diet (ssniff Spezialdiäten GmbH, Ferdinand-Gabriel-Weg, Germany) for 16 weeks.

Techniques: Western Blot, Expressing, Control

Journal: PLoS ONE

Article Title: Effects of vitamin D on insulin resistance and myosteatosis in diet-induced obese mice

doi: 10.1371/journal.pone.0189707

Figure Lengend Snippet: (A) Representative Western blotting analysis for CML-modified proteins and RAGE expression. Protein expression was evaluated on gastrocnemious homogenates of animals fed a control diet or HFHS diet with or without vitamin D supplementation (7 μg/kg, 3 times per week). (B) Densitometric analysis of the bands is expressed as relative optical density (O.D.), corrected for the GADPH contents, and normalized using the related control band. The data aremeans ± S.E.M. of 6–9 randomly selected animals per group. Statistical analysis was performed by One-way analysis of variance with Bonferroni's post-hoc test. *p<0.05, ***p<0.001 vs Control; #p<0.05 vs HFHS.

Article Snippet: The animals were then randomly allocated to two experimental groups: mice fed a control diet or an High Fat-High Sugar (HFHS) diet (ssniff Spezialdiäten GmbH, Ferdinand-Gabriel-Weg, Germany) for 16 weeks.

Techniques: Western Blot, Modification, Expressing, Control